Hello Jeff S.
The program remembers the immunization.
"Simply clicking “Immunize” on the main screen of Anti-Beacon will immediately disable any known tracking features included by Microsoft in the operating system.
If any issues occur with your PC while using Anti-Beacon, undoing the changes made can be done by clicking the “Undo” button in the main window. This will re-enable all tracking services."
https://www.safer-networking.org/spybot-anti-beacon/
Hope that helps.